Business incubators have long been integral to higher education, fostering strong connections between universities and regional business sectors. This comparative case study, part of the HEInnovate initiative, explores the establishment and operation of incubators to support entrepreneurs and innovators in collaboration with higher education institutions (HEIs). The study examines the University College of Borås, TecnoCampus Catalonia/Reimagine Textile, and Foundry Powered by International Fashion Academy (IFA) Paris, utilising public sources and interviews with HEI representatives. The case study highlights how these incubators stimulate entrepreneurship and innovation within the textile industry, emphasising the importance of societal interest in sharing academic knowledge for practical and commercial applications. Strategic alignment to support entrepreneurs is crucial, as demonstrated by collaborations like Beyond Form and IFA Paris, and initiatives such as ‘Urban Agenda Mataró 2030’ at TecnoCampus. External organisations and public institutions play vital roles in launching and managing innovation programs, although cooperation agreements between HEIs and incubators are essential for easing the transition of ideas from academia to the market. The study underscores the importance of these agreements in fostering supportive ecosystems for ventures. Operating innovation programs requires navigating complex regulations while balancing academic and business perspectives. Innovation advisors, such as those at Borås University College, are pivotal in supporting research advancement and business preparation, particularly before market entry.